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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in many tumors, which leads to chromosome instability, and so might add to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important function in advertising mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually explained. Two extra splice variants have been described but in-depth nucleotide sequence info just isn't nonetheless available.
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Blocking peptides are peptides that bind exclusively towards the target antibody and block antibody binding. These peptide ordinarily includes the epitope recognized via the antibody.
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This manual outlines further doable optimization of cellular and immunoassay parameters to ensure the absolute best outcomes are...
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This guideline outlines more doable optimization of mobile and immunoassay parameters to make certain the absolute best success are...
Antibodies bound to the blocking peptide no longer bind for the epitope about the concentrate on protein. By evaluating the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.
Goal Data Cyclin E belongs on the hugely conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E forms a fancy with and functions for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and it is degraded as cells development via S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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